| Group | Club/Team Name | Team ID | Head Coach |
| Seed 1 | Congaree Rapid FC - 01 CRFC Sky 05 Girls PMSL |
02035-0011G7U-0055 | John Collins |
| Seed 2 | CESA Competitive CESA Spartanburg 05G Select |
01001-0011G7U-0023 | Scott Fogarty |
| Seed 3 | James Island Youth SC James Island 2005/06 Girls Black |
03001-0011G7U-0126 | Stewart Garrett |
| Seed 4 | CESA Competitive CESA 05G Select |
01001-0011G7U-0055 | Jose Reyes |
| Seed 5 | Tormenta FC Academy - 01 TFC 05G Magenta |
03035-001CG7U-0124 | James Robbins |
| Seed 6 | Beach United FC - 01 BUFC Panthers U17 |
03022-0011G7U-0130 | Carl Strong |