| Group | Club/Team Name | Team ID | Head Coach |
| Seed 1 | Beach United FC - 01 BUFC Crush U16 |
03022-0011G6U-0132 | Kevin Weidner |
| Seed 2 | Charlotte Soccer Academy 06 CSA Palmetto King G |
02030-0011G6U-0046 | Anthony Gray |
| Seed 3 | South Carolina United FC - Competitive SCUFC 06 Girls Challenge |
02208-2081G6U-0459 | Hannah Baxter |
| Seed 4 | Charleston United SC - 01 Charleston United '06 Girls Blue |
03002-0011G6U-0064 | Ryan Garrett |