| Group | Club/Team Name | Team ID | Head Coach |
| Seed 1 | Cainhoy Athletic Soccer Club CASC 07 Fury |
03006-001CG5U-2243 | Dominique Lopez |
| Seed 2 | CESA Competitive CESA Spartanburg 07G Select |
01001-0011G5U-0031 | Carlos Osorio |
| Seed 3 | Charlotte Independence Soccer Club CISC South YC 07 G Blue |
02014-0011G5U-0621 | Christiana Holz |
| Seed 4 | CESA Competitive CESA 07G Select Black |
01001-0011G5U-0039 | Herman Hassan |
| Seed 5 | Congaree Rapid FC - 01 CRFC Sky 07 Girls PMSL |
02035-0011G5U-0047 | Adam Jordan |
| Seed 6 | CESA Competitive CESA Anderson 07G Select |
01001-0011G5U-0025 | Clinton Gillis |